EpiSC culture medium.

Reagent	Amount	Final concentration
KnockOut DMEM (Gibco#10829–018)	76 mL
KnockOut serum replacement (KSR) (Invitrogen#10828–028)	20 mL	20%
l-Glutamine (200 mM) (Gibco#25030)	1 mL	2 mM
MEM non-essentials amino acids (100 ×) (Gibco#11140–050)	1 mL	1 ×
β-Mercaptoethanol (10 mM) (Sigma#M7522)	1 mL	0.1 mM
FGF2 (R&D Systems) (10 μg/mL)	100 μL	10 ng/μl

Filter and store the medium (final volume 100 mL) at 4°C and use it within 2 weeks. Add FGF2 supplement freshly.