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. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: Curr Top Dev Biol. 2020 Feb 27;138:139–174. doi: 10.1016/bs.ctdb.2020.01.005

BSA fraction V solution in PBS (0.1%, w/v).

Reagent Amount
BSA fraction V (Sigma-Aldrich A3311 or A1470) 10 mg
Phosphate-buffered saline (PBS) without Ca2+/Mg2+ 10 mL

Resuspend BSA in PBS. Filter through a 0.45-μm filter, aliquot, and store at −80°C.