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FGF2 stock solution (10μg/mL).
| Reagent | Amount |
|---|---|
| Recombinant fibroblast growth factor-2 (FGF2; R&D Systems 233-FB-025) | 25 μg |
| BSA fraction V solution in PBS (0.1%, w/v) | 2.5 mL |
Resuspend lyophilized FGF2 in BSA/PBS, mix well, aliquot 50μL, and freeze at −80°C. Thaw each aliquot as needed and store it at 4°C. Do not refreeze the thawed aliquot.
