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. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: Curr Top Dev Biol. 2020 Feb 27;138:139–174. doi: 10.1016/bs.ctdb.2020.01.005

Activin A stock solution (10μg/mL).

Reagent Amount
Recombinant Activin A (ActA; R&D Systems 338-AC-005) 5 μg
BSA fraction V solution in PBS (0.1%, w/v) 0.5 mL

Resuspend lyophilized Activin A in its vial, mix well, aliquot 50μL, and freeze at −80°C. Thaw each aliquot as needed and store it at 4°C. Do not refreeze.