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. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: Curr Top Dev Biol. 2020 Feb 27;138:139–174. doi: 10.1016/bs.ctdb.2020.01.005

CHIR99021 stock solution (10 mM).

Reagent Amount
GSK3 inhibitor CHIR99021 (Stemgent#04–0004) 5 mg
DMSO 1.07 mL

Protect from light, aliquots store at −20°C. The above data is based on the product molecular weight 465.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions. Thaw each aliquot as needed and store it at 4°C. Do not refreeze.