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. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: Curr Top Dev Biol. 2020 Feb 27;138:139–174. doi: 10.1016/bs.ctdb.2020.01.005

MEF medium.

Reagent Amount
MEM Alpha (1 ×) (Gibco#12561–056) 90 mL
FBS (Gibco#10437–028) 10 mL

Filter and store the medium (final volume 100 mL) at 4°C and use within 2 weeks.