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. 2020 May 18;205(1):261–271. doi: 10.4049/jimmunol.1901472

FIGURE 6.

FIGURE 6.

Virus resistance is induced in ISG15-deficient cells following longer periods of IFN-α pretreatment. (A) Experimental workflow. (B) Cells were treated 1000 IU/ml IFN-α in six-well plates for the indicated times prior to infection. Pretreated cells were infected with rPIV5-mCherry (MOI 10) for 48 h, and mCherry fluorescence was measured using an IncuCyte ZOOM. Background fluorescence from mock-infected wells was subtracted. Data are representative to two independent experiments.