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. Author manuscript; available in PMC: 2020 Nov 11.
Published in final edited form as: Nat Chem Biol. 2020 May 11;16(7):731–739. doi: 10.1038/s41589-020-0533-x

Figure 4. G6PDi-1 suppresses T cell cytokine production while having a minimal effect on initial activation or proliferation.

Figure 4.

a, Flow cytometry analysis of cell size (FSCA) and activation markers (CD69 and CD25) of mouse naïve CD8+ T cells either rested in naïve state or stimulated by CD3/CD28 + IL-2 in the presence of increasing concentrations of G6PDi-1. Representative results of 2 independent experiments. b, Proliferation of CD8+ T cells either rested in naïve state or stimulated by CD3/CD28 + IL-2 in the presence of increasing concentrations of G6PDi-1 at day 4 post-activation based on Cell Trace Violet (CTV) dilution. Representative results of 2 independent experiments. c, Intracellular cytokines in active CD8+ T cells from wild-type or G6pd overexpressing mice after a 6 h stimulation with PMA and IO in the presence of the indicated dose of G6PDi-1.(c-d) Representative results of 2 independent experiments. d, Corresponding Ifng mRNA in active CD8+ T cells from wild-type or G6pd overexpression mice (normalized to Gapdh expression and no G6PDi-1 control) (n = 2).