. Author manuscript; available in PMC: 2021 Jul 1.

Published in final edited form as: J Immunol. 2020 May 15;205(1):90–101. doi: 10.4049/jimmunol.1901015

Table 1.

Cloning efficiency and DNA reactivity of B-cell subsets after Nojima cultures¹

Mouse strain	B-cell type	No. Exps.²	Cloning efficiency³	Freq. DNA-binding⁴
B6	Small pre-B	6 (5)	23 (±4.1)	22 (±9.9)
	Immature/T1	6 (4)	56 (±9.6)	20 (±4.8)
	MF	8 (5)	80 (±4.4)	21 (±4.2)
	T3	2 (2)	61 (±2.1)	25 (±9.5)
	CD93⁺ anergic	2 (2)	51 (±2.2)	22 (±3.1)
	CD93⁻ anergic	2 (2)	68 (±6.4)	22 (±1.2)

^1;

Single B cells were sorted from small pre-B, immature/T1 B, MF B, T3 B, and CD93⁺ and CD93⁻ anergic B cells of B6 mice, and cultured in the presence of NB-21.2D9 feeder cells for 9 days. After culture, culture supernatants were harvested for ELISA determinations.

^2;

For each B-cell subset, number of experiments for the determination of cloning efficiency (DNA reactivity) is shown. We determined DNA reactivity from most (77%; 20/26) but not all Nojima cultures.

^3;

Cloning efficiency represents frequency of IgG⁺ samples as a percentage of the number of samples screened. Mean values (±SD) are shown.

^4;

For each B-cell subset, frequency of DNA-binding IgG⁺ samples is shown as a percentage of the number of total IgG⁺ samples. Mean values (±SD) are shown.