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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: FEBS Lett. 2020 Apr 28;594(12):1947–1959. doi: 10.1002/1873-3468.13788

Figure 2: Molecular interactions of LSP1 and BST-2.

Figure 2:

(A & B) BST-2 and LSP1 constructs used for overexpression: HA-tagged BST-2 with GPI mutation by substituting A at position 161 with S (BST-2 ΔGPI-1), BST-2 with GPI mutation by deletion of A at position 161 (BST-2 ΔGPI-2), BST-2 FL (full length), proBST-2; GST-tagged LSP1 N (C-terminal deletion), LSP1 C (N-terminal deletion) and LSP1 FL (full length). (C) Interactions between BST-2 and LSP1 by immunoprecipitation in 293T cells co-transfected with BST-2 FL and LSP1 constructs (N-term, C-term, and FL). BST-2 was used as a loading control. AbC – antibody control; TCL - total cell lysate. (D) Interactions between BST-2 and LSP1 by immunoprecipitation of 293T cells co-transfected with LSP1 N and BST-2 FL, proBST-2, BST-2 ΔGPI-1 and BST-2 ΔGPI-2 constructs. BST-2 was used as a loading control. (E) Co-localization of LSP1 and BST-2 in 293T cells co-transfected with LSP1 N-term and BST-2 FL constructs and stained with anti-GST (green) and anti-HA (red) antibodies. Results are representative of 3 independent experiments. Scale bar = 10μm.