Figure 4.

AMD3100 Overcomes Tamoxifen Resistance via Inhibiting AKT Phosphorylation

(A) p-AKT was significantly downregulated in T47DR cells after T47DR cells were treated with AMD3100 for 24 h. (B) CCK8 assay showed that AMD3100 reversed the resistance of T47DR cells to tamoxifen. (C) Annexin V-FITC/PI assay showed that AMD3100 increased the apoptosis of T47DR cells treated with tamoxifen. (D–G) Compared with the other groups, the TAM + AMD3100-treated mice had significantly smaller tumors, but there was no significant difference in body weight. (H) Compared with the control group, AMD3100 inhibited CXCR4, increased the necrosis of tumor cells, and decreased the expression of Ki67. Differences between groups were analyzed by Student’s t test or two-way ANOVA test. Error bars represent means ± SD of triplicate. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.