Figure 2:

Evaluation of the cellular tumor microenvironment reveals greater suppressive myeloid cells in Py8119 tumors. A) Greater CD45⁺ infiltrates in Py117 than Py8119 tumors after 1×10⁶ cells were inoculated then harvested 10 days after 12 Gy RT treatment would have been given; B) the relative proportion of macrophages was greater in Py8119 tumors. C) The total percentage of inflammatory M1-like CD11b⁺F4/80⁺MHCII⁺iNOS⁺ macrophages remained the same but decreased in Py8119 tumors 10 days after RT. D) The number of M2-like CD11b⁺F4/80⁺Arg1⁺ macrophages were much higher in the Py8119 tumors regardless of RT. E) Alternatively, the tumor microenvironment showed greater numbers of dendritic cells CD11b^-CD11c⁺MHCII⁺ in the sensitive Py117 tumors and was nearly absent in Py8119 tumors. F) The macrophage-promoting growth factor CSF1 and chemokines CCL2, CCL5, CCL7, CCL3, and CXCL10 were elevated in Py8119 tumors and were significantly induced after RT, as measured by the Luminex multiplex assay. * <0.01, ** <0.0001, *** <0.001, and **** <0.05 on analysis of variance with pairwise comparisons.