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. 2020 Jun 23;10:10202. doi: 10.1038/s41598-020-67087-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Cyclic Mechanical Stress on the Status of RhoA activation. Western blot analysis of (A) active RhoA, total RhoA and (B) RhoA downstream effector protein p-MLC. There was a significant increase in activated RhoA (p = 0.002) as well as p-MLC (p = 0.006) in TM tissue lysates obtained from the pulsed anterior segment as compared to its control. Levels of activated RhoA were normalized to total RhoA protein levels. p-MLC immunoblots were normalized to the total MLC loading control. The cropped images are used in the figure, and full length blots for the same are presented in S. Figure 5A,B. Individual data points (black color dots) are indicated on the bar graph representing mean ± SD. **p < 0.005; *p < 0.05; Student’s t-test; n = 3.