Table 1.
Summary of Results from HOCAS in response to cyclic pulsation in the presence or absence of SB77.
| Experimental Setup 1 - Cyclic Pulsation and Steady state flow comparison | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor Code | Age | Sex | Stable Baseline Before Pulse (µl/minute/mm Hg) | Pulse Duration (h) | Outflow Facility (24 h) (Normalized to Baseline) | OFR (EXP/CTL) | % Change in OF | ||||
| Control (no pulse) | Cyclic pulse | Control (no pulse) | Cyclic pulse | ||||||||
| 1 | 75 | M | 0.17 | 0.25 | 8 | 0.94 | 0.88 | 0.95 | −5.44 | ||
| 2 | 40 | M | 0.21 | 0.28 | 8 | 1.07 | 0.64 | 0.6 | −39.92 | ||
| 3 | 60 | M | 0.08 | 0.19 | 8 | 0.95 | 0.84 | 0.88 | −11.62 | ||
| 4 | 85 | F | 0.14 | 0.26 | 8 | 0.8 | 0.68 | 0.85 | −14.92 | ||
| 5 | 72 | M | 0.08 | 0.16 | 8 | 0.77 | 0.58 | 0.76 | −24.23 | ||
| 6 | 53 | F | 0.29 | 0.2 | 8 | 0.85 | 0.65 | 0.77 | −22.66 | ||
| 0.89 ± 0.04 | 0.71 ± 0.05 | −19.8 ± 4.9 | |||||||||
| Experimental Setup 2 - Cyclic Pulsation in Presence or Absence of SB77 | |||||||||||
| Donor Code | Age | Sex | Stable Baseline Before Pulse (µl/minute/mm Hg) | Pulse Duration (h) | Outflow Facility (24 h) (Normalized to Baseline) | % Change in OF | |||||
| Cyclic pulse | Cyclic pulse + SB77 | Cyclic pulse | Cyclic pulse + SB77 | Cyclic pulse | Cyclic pulse + SB77 | ||||||
| 1 | 72 | M | 0.15 | 0.19 | 8 | 0.85 | 1.27 | −15.35 | 27.01 | ||
| 2 | 85 | M | 0.18 | 0.14 | 8 | 0.89 | 1.17 | −10.53 | 16.67 | ||
| 3 | 70 | M | 0.09 | 0.14 | 8 | 0.91 | 1.11 | −8.65 | 11.2 | ||
| 4 | 85 | F | 0.27 | 0.12 | 8 | 0.8 | 1.03 | −19.95 | 2.82 | ||
| 5 | 81 | M | 0.08 | 0.08 | 8 | 0.74 | 1.19 | −25.9 | 19.44 | ||
| 0.84 ± 0.06 | 1.15 ± 0.09 | −16.1 ± 7.0 | 15.4 ± 9.06* | ||||||||
Outflow facility (OF) (μl/minute/mm Hg) was calculated as the ratio between the inflow rate (μl/minute) and the measured IOP (mm Hg). OFR was calculated using the formula: Treated/BL/Control/BL. % Change in OF was calculated using the formula: [(Treated/BL/Control/BL) -1] X100.
An average decrease in outflow facility (−19.79 ± 4.93%; n = 6) was observed in response to IOP oscillations (experimental setup1). In another set of experiments, an overall decrease in outflow facility of −16 ± 7.0% (n = 5) was observed in anterior segments those received IOP pulsations. Treatment with 50 µM SB77 caused significant enhancement in outflow facility by 15% (p < 0.05) (experimental setup 2). Data are represented as Mean ± SEM; **p < 0.005; *p < 0.05; Paired-t-test. EXP – Experiment; CTL = Control; OFR – Outflow ratio.