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. 2020 Jun 23;10:10154. doi: 10.1038/s41598-020-67103-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence staining of scrotal skin in control (A & C) and melatonin group (B & D). Merged immunofluorescence for S100 (green) and PGP (red) in control group (A) melatonin group (B), inner cells of sebaceous glands (arrowheads) in melatonin treated group showing positive staining for S100 (green). Notice, colocalization of PGP and S100 (yellow) was exhibited in TC (wavy arrow) which surrounding the sebaceous gland in melatonin treated group. Immunofluorescence for synaptophysin in the sweat gland showing strong positive reactivity of the secretory granules in the melatonin group (D) incomparable to the control (C).