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. 2020 Jun 23;10:10154. doi: 10.1038/s41598-020-67103-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sebaceous gland, sweat gland, telocytes, and dendritic cells on control and treated groups. (A–F) Sebaceous gland (SB) showing hypertrophy and hyperplasia in treated group, and the sweat gland (SW) were more numerous in the treated group (A: PAS-Hx. F: Hx-E). (B–G) Hx-E staining showing sebaceous gland (SB) and basal cells (black arrows), which appeared larger and arranged on several layers on the treated group. (C–H) Hx-E staining showing sweet gland (SW) with well-developed secretory activity in melatonin group in compared with control one. (D-E-I-J) Telocytes (black arrows), blood vessel (BV), epidermis (Ep) and dermis (D). (D) Grimelius silver nitrate method. (E) PAS-Hx. (I) Hx-E. (J) Crossmon’s trichrome staining). (K) Grimelius silver nitrate method showing dendritic cell on dermis of treated group (black arrows).