Table 1.
Identity, sources, and working dilution of antibodies used in immunohistochemical and immunofluorescence studies.
| Primary antibodies | Primary antibody Supplier (Catalog No.) | Origin | Dilution | Biotinylated secondary antibody (IHC) |
|---|---|---|---|---|
| 1.Anti-S100 protein | Dako, #Z0311 | Rabbit polyclonal |
1:500 for IHC 1:200 for IF |
Goat anti rabbit IgG) |
| 2.PGP 9.5 | Sigma immune chemicals# MCA-BH7 | Mouse monoclonal |
1:2000 for IHC 1:200 for IF |
Goat anti mouse IgG) |
| 3.AntiCytokeratin | Sigma immune chemicals# c7159 | Mouse monoclonal | 1:200 | Goat anti mouse IgG |
| 4.SNAP 25 | Santa cruz biotechnology (sc-20038) | Mouse monoclonal | 1:200 | Goat anti mouse IgG |
| 5.VAMP-1/2 | Santa cruz biotechnology (sc-20039) | Mouse monoclonal | 1:200 | Goat anti mouse IgG |
| 6. Anti-Keratin 5 | Sigma immune chemicals# SAB4501651 | Rabbit polyclonal | 1:500 | Goat anti rabbit IgG) |
| 7. MHC II | Abcam (ab 23990) | Mouse monoclonal | 1:100 | Goat anti mouse IgG |
| 8. Anti-Synaptophysin | Sigma immune chemicals# S5768 | Mouse monoclonal | 1:200 | — |