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. 2020 Jun 23;11:3183. doi: 10.1038/s41467-020-16920-3

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© The Author(s) 2020

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Fig. 3 — a Using AS-MS, the same population of compounds can be used in selections against more than one target in parallel, enabling assessment of target-dependent enrichment of peptides. Here, selections using a 2 × 10⁸-member library were performed against 12ca5 and a polyclonal human IgG₁ in parallel, followed by sequential nLC-MS/MS analysis. b Extracted ion chromatograms of a DXXDY(A/S)-containing peptide (left) and a non-motif-containing peptide (right) from a selection against 12ca5 (blue) and polyclonal human IgG₁ (purple). The peptide containing the 12ca5-binding motif is selectively enriched towards 12ca5, while the non-motif-containing peptide is promiscuously pulled down in both conditions.