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. 2020 Jun 23;11:3165. doi: 10.1038/s41467-020-16983-2

Fig. 2. SAMHD1 tetramerisation and hydrolysis of dNMPCPP analogues.

Fig. 2

a SEC-MALLS analysis of SAMHD1 monomer–dimer–tetramer equilibrium upon addition of dNMPCPP nucleotides. In each panel, the solid lines are the chromatograms from the output of the differential refractometer and the black scatter points are the weight-averaged molar masses determined at 1-s intervals throughout elution of chromatographic peaks. In each panel, the displayed curves are: (red) apo-SAMHD1; (blue) SAMHD1 and 0.2 mM GTP; and (black) SAMHD1, 0.2 mM GTP and 0.5 mM indicated dNMPCPP analogue. b Bar chart of the apparent kcat values derived from 1H NMR data recorded for GTP-stimulated SAMHD1 hydrolysis of each dNTP, dNMPCPP and dNMPCPP/dATP reaction, see Supplementary Fig. 3. Points overlaid in each bar are the individual data measurements, the error bars represent the standard deviation (s.d.) from at least two independent measurements. The source data are provided in the Source Data file.