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. 2020 Jun 23;11:3181. doi: 10.1038/s41467-020-16997-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Scheme of the genetic system to test ectopic gene conversion (eGC) in tGI354 background. b Cell viability after DSB in the indicated tGI354 derivative strains (n = 3 independent experiments) combined with the frequency of crossovers (CO) and non-crossovers (NCO) in the survivors measured by PCR analysis. c Southern blot of EcoRI-digested DNA to monitor DSB repair through eGC in the indicated tGI354 derivative strains. CO crossover, NCO non-crossover. d Densitometric analysis of the CO and NCO bands of two biologically independent experiments as in (c). All the data in the figure are presented as mean ± SEM wherever n = 3 or more. Statistical analysis was performed using unpaired two-tailed Student’s t-test. See accompanying Source data file.