. 2020 Jun 23;10:10201. doi: 10.1038/s41598-020-67260-7

Table 1.

Effects of waterlogging at the seedling stage and nitrogen application on the accumulation of dry matter in the shoot of winter rapeseed.

Water logging	N level	Pot experiment (g/plant)				Field experiment (kg/hm²)
Water logging	N level	0day	Budding Stage	Flowering Stage	Maturity Stage	0day	Budding Stage	Flowering Stage	Maturity Stage
D0	N1	3.41 ± 0.10^a	12.90 ± 0.53^c	40.89 ± 2.67 ^cd	41.34 ± 1.32^e	310.33 ± 12.14^ab	1591.67 ± 37.31^c	3977.00 ± 268.97 ^cd	5201.00 ± 309.31^de
	N2	3.53 ± 0.02^a	14.73 ± 0.24^a	45.47 ± 2.66^bc	52.71 ± 3.92^c	313.83 ± 5.78^a	1775.00 ± 39.09^a	4543.38 ± 302.14^abc	7907.00 ± 380.72^b
	N3	3.46 ± 0.10^a	13.86 ± 0.24^b	52.60 ± 2.82^a	70.58 ± 1.25^a	291.67 ± 6.92^abc	1836.50 ± 29.77^a	4849.00 ± 153.95^a	8737.00 ± 349.93^a
	N4	3.43 ± 0.22^a	13.44 ± 0.42^bc	50.62 ± 2.54^ab	61.78 ± 0.74^b	315.83 ± 12.68^a	1681.50 ± 35.06^b	4623.67 ± 258.83^ab	6267.00 ± 110.78^c
D6	N1	2.64 ± 0.04^b	9.41 ± 0.09 ^f	32.08 ± 0.10^ef	30.24 ± 1.47^fg	280.67 ± 12.23^bcd	926.50 ± 18.50 ^h	2328.50 ± 39.91 ^g	3036.00 ± 220.46^fg
	N2	2.62 ± 0.13^b	11.33 ± 0.11^de	36.98 ± 0.50^de	35.29 ± 1.48 ^f	257.33 ± 8.59^d	1154.83 ± 30.08 ^f	3396.83 ± 198.82^ef	4543.50 ± 210.91^e
	N3	2.51 ± 0.16^b	14.00 ± 0.17^ab	45.05 ± 3.12^bc	46.64 ± 0.86^d	272.33 ± 8.67 ^cd	1392.33 ± 23.64^d	4116.83 ± 127.68^bcd	5495.50 ± 129.01^d
	N4	2.44 ± 0.09^b	11.78 ± 0.20^d	40.95 ± 2.12 ^cd	40.96 ± 0.63^e	269.33 ± 11.57 ^cd	1283.00 ± 34.88^e	3738.67 ± 189.05^de	5243.50 ± 94.91^de
D9	N1	2.00 ± 0.15^c	6.55 ± 0.06 ^h	18.39 ± 1.19 ^g	23.75 ± 1.20 ^h	172.67 ± 13.72^e	577.00 ± 15.60^j	1718.00 ± 20.23 ^h	2062.50 ± 180.60 ^h
	N2	2.09 ± 0.04^c	8.07 ± 0.07 ^g	29.61 ± 1.49 ^f	28.27 ± 1.34^gh	155.17 ± 5.78^e	757.00 ± 23.88ⁱ	2217.33 ± 137.51^gh	2740.00 ± 201.73 ^g
	N3	2.05 ± 0.03^c	9.34 ± 0.27 ^f	34.28 ± 1.99^def	31.14 ± 0.21^fg	148.50 ± 10.75^e	1024.17 ± 34.61 ^g	2963.17 ± 143.89 ^f	3171.50 ± 31.28^fg
	N4	2.01 ± 0.05^c	10.69 ± 0.15^e	35.39 ± 2.71^def	34.71 ± 1.76 ^f	163.00 ± 6.66^e	1104.67 ± 15.61^fg	3108.17 ± 175.51 ^f	3707.00 ± 263.51 ^f

Values are means of three replicates ±SE. Values followed by the similar lower case letters within columns for waterlogging treatments don’t differ significantly according to Duncan’s test (P ≤ 0.05).The different application levels of nitrogen were N1 (0 kg ha⁻¹), N2 (30 kg ha⁻¹), N3 (60 kg ha⁻¹) and N4 (90 kg ha⁻¹). D0, refers to without waterlogging; D6, refers to waterlogging for 6 days and D9, refers to waterlogging for 9 days. 0 day refers to the days after nitrogen application.