. 2020 Jun 23;10:10201. doi: 10.1038/s41598-020-67260-7

Table 2.

Effects of waterlogging at the seedling stage and nitrogen application on the accumulation of dry matter in the root of winter rapeseed.

Water logging	N level	Pot experiment (g/plant)				Field experiment (kg/hm²)
Water logging	N level	0day	Budding Stage	Flowering Stage	Maturity Stage	0day	Budding Stage	Flowering Stage	Maturity Stage
D0	N1	0.44 ± 0.03^a	3.63 ± 0.05^b	6.58 ± 0.11^c	7.47 ± 0.11^e	41.42 ± 3.96^ab	371.67 ± 11.18^b	626.33 ± 13.72^b	780.50 ± 9.85^c
	N2	0.45 ± 0.02^a	4.63 ± 0.13^a	7.61 ± 0.09^b	10.66 ± 0.31^c	46.00 ± 3.88^a	475.00 ± 78.49^a	790.00 ± 24.27^a	1180.00 ± 31.82^b
	N3	0.43 ± 0.01^a	4.50 ± 0.08^a	8.02 ± 0.25^a	12.71 ± 0.17^a	40.00 ± 2.52^ab	489.17 ± 17.73^a	766.17 ± 8.17^a	1277.00 ± 37.97^a
	N4	0.42 ± 0.01^a	4.45 ± 0.03^a	7.94 ± 0.05^ab	11.11 ± 0.09^b	45.17 ± 9.68^a	465.50 ± 21.30^a	753.33 ± 25.13^a	1158.50 ± 35.38^b
D6	N1	0.34 ± 0.01^b	2.41 ± 0.07^e	5.08 ± 0.07^e	5.43 ± 0.05 ^h	32.33 ± 1.30^bc	256.00 ± 26.10^def	418.33 ± 11.09^d	476.50 ± 17.52^fg
	N2	0.33 ± 0.01^b	3.20 ± 0.05^c	5.92 ± 0.13^d	6.47 ± 0.16 ^f	27.17 ± 0.88^cde	300.33 ± 16.10^bcd	481.17 ± 10.17^c	658.00 ± 34.97^d
	N3	0.32 ± 0.02^b	3.73 ± 0.04^b	6.69 ± 0.06^c	7.97 ± 0.08^d	32.67 ± 1.36^bc	379.17 ± 8.08^b	638.67 ± 15.28^b	822.00 ± 21.70^c
	N4	0.30 ± 0.01^bc	3.71 ± 0.13^b	6.11 ± 0.03^d	6.56 ± 0.04 ^f	28.17 ± 1.30 ^cd	356.50 ± 12.77^bc	622.67 ± 8.80^b	762.50 ± 11.95^c
D9	N1	0.23 ± 0.02^d	1.73 ± 0.08 ^g	3.12 ± 0.10 ^g	3.84 ± 0.11^j	23.17 ± 2.20^cde	183.50 ± 9.73 ^f	257.17 ± 10.65 ^f	302.00 ± 20.90 ^h
	N2	0.26 ± 0.02 ^cd	2.13 ± 0.05 ^f	4.55 ± 0.07 ^f	4.76 ± 0.08ⁱ	18.17 ± 1.20^d	207.33 ± 21.18^ef	340.33 ± 3.56^e	423.00 ± 17.90 ^g
	N3	0.25 ± 0.01 ^cd	2.57 ± 0.07^de	5.14 ± 0.12^e	5.58 ± 0.10^gh	16.50 ± 1.04^e	270.17 ± 14.42^cdef	421.67 ± 11.92^d	542.50 ± 16.26^ef
	N4	0.23 ± 0.01^d	2.71 ± 0.03^d	5.21 ± 0.15^e	5.91 ± 0.08 ^g	18.50 ± 2.36^de	290.83 ± 9.28^bcde	455.83 ± 6.77 ^cd	585.50 ± 15.88^e

Values are means of three replicates ±SE. Values followed by the similar lower case letters within columns for waterlogging treatments don’t differ significantly according to Duncan’s test (P ≤ 0.05).The different application levels of nitrogen were N1 (0 kg ha⁻¹), N2 (30 kg ha⁻¹), N3 (60 kg ha⁻¹) and N4 (90 kg ha⁻¹). D0, refers to without waterlogging; D6, refers to waterlogging for 6 days and D9, refers to waterlogging for 9 days. 0 day refers to the days after nitrogen application.