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. 2020 Jun 23;11:3168. doi: 10.1038/s41467-020-16971-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Reconstructed CT-scanned images of vertebrae illustrate the measured features (a–c): The ratios between anterior (dashed line indicated by arrowhead) and posterior (dashed line indicated by arrow) heights (a, sagittal plane, A/P ratio), between right (arrowhead) and left (arrow) heights (b, coronal plane, R/L ratio) and between right (dashed circle indicated by arrowhead) and left (dashed circle) superior facet angles (c, transverse plane, R/L facet angle ratio). Graphs below show the morphometric similarity between PValb-Cre;Piezo2^f/f (dark gray) and control (light gray) mice (n = 5 in each group). A/P anterior/posterior, R/L right/left. d Graph showing the 95% confidence intervals for differences in mean ratios. Red and green dots indicate upper and lower confidence limits, respectively, and black dots indicate the difference between control and mutant means ratio, which did not exceed the −0.1 to 0.1 range in all indices and at all levels. Data are presented as mean ± SEM; n_control = 5, n_cKO = 5. Source data are provided as a Source Data file.