Table 1.
Mutation status of crucial genes and the total number of differentially methylated (DM) CpGs with Δβ value ≥0.2 or ≤−0.2 in KRAS-depleted pancreatic cancer cell lines.
| Cell Line | KRAS | CDKN2A | TP53 | SMAD4 | Hypermethylated Promoter CpGs/total CpGs | Hypomethylated Promoter CpGs/total CpGs | All CpGs with Δβ ≥0.2 or ≤−0.2 |
|---|---|---|---|---|---|---|---|
| Pa16C | G12D/WT | I255N* | 434/3275 | 764/5613 | 8888 | ||
| Pa01C | G12D/WT | T155P* | Del* | 175/1248 | 393/2998 | 4246 | |
| PANC-1 | G12D/WT | Del* | R273H* | 128/717 | 556/3136 | 3853 | |
| Panc 10.05 | G12D/WT | I255N/WT | 59/452 | 275/1508 | 1960 | ||
| Pa04C | G12V* | Del* | Del* | 13/172 | 25/200 | 372 | |
| Pa02C | Q61H* | Del* | L247P* | Del* | 28/185 | 28/184 | 369 |
| CFPAC-1 | G12V/WT | C242R* | Del* | 7/89 | 12/115 | 204 | |
| HPAC | G12D* | Stop/Stop | 16/104 | 15/92 | 196 | ||
| HPAF-II | G12D/WT | Del-FS* | P151S* | 14/96 | 5/68 | 164 | |
| SW-1990 | G12D* | Del* | 11/78 | 15/79 | 157 | ||
| Pa18C | G12D/WT | Del* | Del* | 6/72 | 6/72 | 144 |
The CpG methylation in Pa16C, Pa01C, PANC-1 and Panc 10.05 cells appears to be the most responsive to KRAS depletion. Homozygous mutations are represented with an asterisk.