Table 1.
The table shows the IHC, PCR and IR imaging results for the 60 independent validation patients.
| Patient | MLH1% | MSH2% | MSH6% | PMS2% | MSI-FLA | BAT25 | BAT26 | D17S250 | D2S123 | D5S346 | IR imaging | IR MSI-H positive % |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| V01 | 80 | 90 | 85 | 80 | IHC + | MSS | 62,38 | |||||
| V02 | 20 | 90 | 60 | 50 | IHC + | MSS | 39,87 | |||||
| V03 | 90 | 85 | 95 | 85 | IHC + | MSS | 52,30 | |||||
| V04 | 80 | 85 | 70 | 65 | IHC + | MSS | 60,78 | |||||
| V05 | 80 | 70 | 50 | 60 | IHC + | MSS | 43,35 | |||||
| V06 | 95 | 95 | 60 | 75 | IHC + | MSS | 56,04 | |||||
| V07 | 95 | 95 | 70 | 85 | IHC + | MSS | 58,90 | |||||
| V08 | 90 | 90 | 90 | 90 | IHC + | MSS | 59,04 | |||||
| V09 | 90 | 90 | 50 | 70 | IHC + | MSS | 54,99 | |||||
| V10 | 95 | 90 | 60 | 85 | IHC + | MSS | 46,81 | |||||
| V11 | 95 | 95 | 90 | 80 | IHC + | MSS | 47,30 | |||||
| V12 | 90 | 90 | 60 | 80 | IHC + | MSS | 60,14 | |||||
| V13 | 80 | 90 | 80 | 90 | IHC + | MSS | 51,45 | |||||
| V14 | 90 | 95 | 70 | 80 | IHC + | MSS | 54,20 | |||||
| V15 | 90 | 95 | 90 | 90 | IHC + | MSS | 58,29 | |||||
| V16 | 80 | 80 | 75 | 80 | IHC + | MSS | 50,12 | |||||
| V17 | 90 | 95 | 30 | 90 | IHC + | MSS | 54,28 | |||||
| V18 | 75 | 40 | 80 | 85 | IHC + | MSS | 58,51 | |||||
| V19 | 80 | 85 | 60 | 80 | IHC + | MSS | 55,87 | |||||
| V20 | 90 | 90 | 90 | 70 | IHC + | MSI-H | 68,33 | |||||
| V21 | 95 | 95 | 90 | 90 | IHC + | MSS | 51,54 | |||||
| V22 | 85 | 90 | 90 | 85 | IHC + | MSS | 59,23 | |||||
| V23 | 80 | 90 | 60 | 75 | IHC + | MSS | 41,15 | |||||
| V24 | 75 | 80 | 70 | 75 | IHC + | MSS | 61,76 | |||||
| V25 | 90 | 95 | 75 | 70 | IHC + | MSS | 60,49 | |||||
| V26 | 85 | 90 | 40 | 75 | IHC + | MSS | 60,87 | |||||
| V27 | 95 | 90 | 70 | 85 | IHC + | MSS | 54,94 | |||||
| V28 | 60 | 80 | 25 | 55 | IHC + | MSS | 39,29 | |||||
| V29 | 85 | 90 | 90 | 80 | IHC + | MSS | 39,4 | |||||
| V30 | 80 | 95 | 90 | 80 | IHC + | MSI-H | 73,23 | |||||
| V31 | 5 | 90 | 90 | 0 | MSI-H | is | is | is | is | is | MSI-H | 80,91 |
| V32 | 0 | 90 | 90 | 0 | MSI-H | is | is | is | is | is | MSI-H | 70,99 |
| V33 | 1 | 85 | 70 | 1 | MSI-H | is | is | is | is | is | MSI-H | 81,24 |
| V34 | 2 | 90 | 90 | 1 | MSI-H | is | is | is | is | is | MSI-H | 72,12 |
| V35 | 95 | 98 | 85 | 2 | MSI-H | is | is | is | is | is | MSI-H | 65,67 |
| V36 | 1 | 90 | 80 | 1 | MSI-H | is | is | s | is | is | MSI-H | 75,18 |
| V37 | 1 | 70 | 60 | 1 | MSI-H | is | is | is | is | is | MSI-H | 67,41 |
| V38 | 95 | 1 | 1 | 90 | MSI-H | is | is | s | is | is | MSI-H | 65,45 |
| V39 | 1 | 90 | 80 | 1 | MSI-H | is | is | is | is | is | MSI-H | 79,00 |
| V40 | 1 | 90 | 90 | 5 | MSI-H | is | is | NA | is | NA | MSI-H | 96,94 |
| V41 | 1 | 60 | 80 | 5 | MSI-H | is | is | is | is | is | MSI-H | 66,94 |
| V42 | 1 | 90 | 65 | 5 | MSI-H | is | is | is | is | s | MSI-H | 81,71 |
| V43 | 2 | 60 | 50 | 1 | MSI-H | is | is | is | s | s | MSI-H | 76,46 |
| V44 | 1 | 85 | 70 | 0 | MSI-H | is | is | ND | ND | ND | MSI-H | 87,92 |
| V45 | 2 | 90 | 95 | 1 | MSI-H | is | is | is | is | is | MSI-H | 76,30 |
| V46 | 0 | 95 | 90 | 0 | MSI-H | is | is | is | is | is | MSI-H | 88,93 |
| V47 | 1 | 90 | 85 | 1 | MSI-H | is | is | ND | ND | ND | MSI-H | 89,47 |
| V48 | 10 | 90 | 70 | 10 | MSI-H | is | is | is | is | s | MSI-H | 73,45 |
| V49 | 1 | 90 | 80 | 1 | MSI-H | is | is | is | is | is | MSI-H | 86,46 |
| V50 | 1 | 95 | 65 | 1 | MSI-H | is | is | is | is | is | MSI-H | 81,49 |
| V51 | 1 | 45 | 20 | 1 | MSI-H | is | is | is | is | is | MSI-H | 66,95 |
| V52 | 1 | 70 | 50 | 1 | MSI-H | is | is | is | is | is | MSI-H | 84,33 |
| V53 | 1 | 98 | 80 | 2 | MSI-H | is | is | is | is | is | MSI-H | 82,68 |
| V54 | 1 | 85 | 70 | 1 | MSI-H | is | is | is | is | is | MSI-H | 69,04 |
| V55 | 1 | 90 | 70 | 1 | MSI-H | is | is | is | is | is | MSI-H | 81,61 |
| V56 | 1 | 90 | 80 | 1 | MSI-H | is | is | is | is | is | MSI-H | 73,24 |
| V57 | 1 | 80 | 25 | 1 | MSI-H | is | is | is | is | is | MSI-H | 71,72 |
| V58 | 5 | 95 | 95 | 2 | MSI-H | s | is | is | is | s | MSI-H | 63,54 |
| V59 | 2 | 90 | 85 | 5 | MSI-H | is | is | is | is | s | MSI-H | 64,60 |
| V60 | 0 | 90 | 75 | 1 | MSI-H | is | is | is | is | is | MSI-H | 71,96 |
Two of the 30 MSS patients were misclassified. (Abbreviations: FLA – fragment length analysis, IHC + - no missing MMR proteins, is – instable, s – stable, NA – DNA quality insufficient, ND – not done following Bethesda guidelines, when BAT25 + BAT26 instable).