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MiaPaCa-2 spheroids growth using different methods. The hanging drop and the round bottom well methods were tested both with simple media (A,C) and with MC-enriched media (B,D) to increase media viscosity and thus to improve cellular aggregation. Cell-embedded Matrigel method was carried out in standard 96 well-plates without the addition of MC (E). Spheroids were made of 30.000 cells per spheroid. Assembly time was 14 days for all methods. Bars = 1000 µm.
