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. 2020 Jun 23;10:10192. doi: 10.1038/s41598-020-66908-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of complete (A) and partial (B) media changing on spheroids. Without MC, the partial media changing allowed to preserve spheroids, but they disaggregated at edge level (panel C); this did not occur in presence of MC in the media (panel E). Panels D, F are zoomed areas of dashed boxes in panels C and E, respectively. Bars in panels A, B: 1000 µm; bars in panels C, E: 400 µm; bars in panels D, F: 200 µm.