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. 2020 Jun 23;10:10192. doi: 10.1038/s41598-020-66908-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Live/dead assay on spheroids grown with hanging drop. Representative CLSM images of hanging drop-based spheroids without MC (A,B) and with MC (C,D external regions; E,F internal regions) stained for live (calcein AM; 3 μM solution; in green) and dead (propidium iodide; 10 μM solution; in red) cells after 14 days. Bars = 100 µm.