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. 2020 Jun 23;10:10192. doi: 10.1038/s41598-020-66908-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development of hanging drop-based spheroids on substrate with different hydrophobicity. Drop of 20 μl of media containing 30.000 cells were placed on (A) glass coverslip, (B) PDMS monolayer and (C) standard Petri lid. Spheroids grown on these substrates were monitored up to 10 days. Bars = 1000 µm (4X objective lens).