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. 2020 Jun 23;10:10192. doi: 10.1038/s41598-020-66908-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spheroid growth characterization in relation to the substrate used. (A) Pictures and schematic representation of hanging drop cultures performed on glass, PDMS and Petri lid substrates. (B,C,D): analysis of diameter, aspect ratio and % of successful spheroids at day 10, respectively. One-way between-group ANOVA test (p < 0.10) was used to determine the statistical differences between the different categories for each time point.