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. 2020 Jun 23;11:3173. doi: 10.1038/s41467-020-16902-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a In vivo imaging and immunostaining of retinas 4 h post-RVO or sham laser (white = isolectin, red = cl-caspase-9, green = caspase-7, n = 4, scale bar = 500 μm, burn sites indicated to scale on fundus and IHC imaging; teal oval = laser burn + occlusion, magenta oval = laser burn/no occlusion). b Closeups of immunostaining in (a), at sites distal to the location of laser application (scale bar = 50 μm). c Quantification of cl-caspase-9 and caspase-7 signal intensity in (a); two-tailed Welch’s t test; mean ± SEM. d Correlation of cl-caspase-9 and caspase-7 signal intensity in (a) (linear regression). cl-casp9, cl-caspase-9; casp7, caspase-7. Source data are provided as a Source Data file.