Table 1.
Primer sequences and amounts used in the allele-specific PCRs
| Primer | pmol/25 μl | Sequence | Amplicon length, bp |
|---|---|---|---|
| FVL 1691G | 5 |
5′-AACAAGGACAAAATACCTGTAT- TCATC-3′ |
233 |
| FVL 1691A | 5 |
5′-GTCTGTCTGTCTCTTCAAGGAC- AAAATACCTGTATTCTTT-3′ |
246 |
| FVL common | 5 | 5′-CGCAGGAACAACACCATGAT-3′ | |
| FII 20210G | 2.5 | 5′-CACTGGGAGCATTGAGGCGC-3′ | 180 |
| FII 20210A | 10 |
5′-ATGAATAGTAATGGGAGCATT- GAGGATT-3′ |
188 |
| FII common | 10 |
5′-ATGTGTTCCGCCTGAAGAAGT- GGA-3′ |
|
| MTHFR 677C | 4 |
5′-CTCTCTCTCTGAAGGAGAAGGT- GTCTGCGGTAGC-3′ |
207 |
| MTHFR 677 T | 5 |
5′-TGAAGGAGAAGGTGTCTGCGG- GACT-3′ |
198 |
| MTHFR common | 6 | 5′-AGGACGGTGCGGTGAGAGTG-3′ | |
| PAI-1 F | 1 | 5′-CACAGAGAGAGTCTGGCCACGT-3′ | / |
| PAI-1 R | 1 | 5′-CCAACAGAGGACTCTTGGTCT-3′ | / |
PCR, polymerase chain reaction; FV, factor V; FII, factor II; MTHFR, methylenetetrahydrofolate reductase; PAI, plasminogen activator inhibitor