Figure 5.

Eomes upregulation is correlated with Group 1 ILC-mediated cytotoxicity. (A) Representation of IFNγ expression in Eomes^lo and Eomes^hi subsets relative to FMO controls; red—Eomes^lo, black—Eomes^hi; Cells were isolated and stimulated ex vivo with PMA and Ionomycin (NS = non-stimulated) (B) Increase in Eomes^lo and Eomes^hi cell numbers in response to IL-12 and IL-18 stimulation in vivo. The lungs were harvested, and cells were counted at day 7. Here, n = 4 biological replicates. (C) Percentage killing of B16F10 melanoma cells by Eomes^hi subset relative to Eomes^lo cells at 24 and 48 h, normalized to spontaneous death (B16F10 only). (D) Measurement of Eomes MFI across 24, 48, and 72 h in flow-sorted murine Eomes^hi Group 1 ILC in co-culture with B16F10 cells compared to Eomes^hi subset alone. Cells were isolated from Eomes-GFP reporter mice; Eomes MFI is indicative of GFP MFI in the cells. Target to Effector ratio (T:E) was maintained at 4:1. MFI, Mean Fluorescence Intensity, n = 3 technical replicates, Data are representative of three independent repeats; data are presented as mean ± s.e.m.; Significance was tested using two-tailed students' t-test; n.s., not significant *p < 0.05.