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Production of hydrogen from NADH by HydAB independent of reduced ferredoxin. The enzyme assay was performed with 50.7 μg HydAB (+HydAB). NADH was added to a final concentration of 1 mM (+NADH). The maximal rate of hydrogen production in the enzyme assay was 30.1 nanomoles ⋅ min–1 ⋅ mg–1.
