Skip to main content
. 2020 Jun 17;11:879. doi: 10.3389/fphar.2020.00879

Figure 3.

Figure 3

BA inhibited breast cancer migration and invasion via NF-κB signaling pathway. (A) Migrations of MDA-MB-231 and 4T1 cells were measured by wound-healing assay after treated with different concentrations (0, 5, 10, and 20 μM) of BA for 48 h. (B) Invasions of MDA-MB-231 and 4T1 cells were examined by transwell assay after different concentrations (0, 5, 10, and 20 μM) of BA for 48 h. (C–F) Changes of NF-κB and EMT related proteins in MDA-MB-231 and 4T1 cells were evaluated by western-blot assay after different concentrations (0, 5, 10, and 20 μM) of BA for 48 h. (G) Immunofluorescent analysis of nuclear transportation of NF-κB p65 protein in HCT116 cell. Cells were exposed to 20 μM of BA for 24 h and/or 15 ng/ml of TNF-α for 4 h. Significant differences are indicated as follows: **P < 0.01; ***P < 0.001.