Figure 5.

BA suppressed 4T1 tumor growth by inhibiting NF-κB signaling pathway. 1×10⁶ 4T1 cells which was suspended in 100 μl of serum-free cell culture medium were injected into right flank of female Balb/c mouse to establish tumor mouse model. When the tumor volume reached about 100 mm³, the tumor-bearing mice were intraperitoneally administrated with vehicle, 25 mg/kg, or 50 mg/kg of BA every 2 days. (A) Curve of tumor growth during the process of experiment. (B) Tumor images of different treated groups at the termination of animal experiment in the tumor mouse model of 4T1 cells. (C) Tumor weight of different treated groups at the termination of animal experiment in the tumor mouse model of 4T1 cells. (D) Body weight of mice of different treated groups during the process of experiment. (E) Pathological analysis of major organs of subcutaneous tumor-bearing mice after treatment of different doses of baicalin. (F) Immunohistochemical analysis of Ki67, Cleaved caspase3, NF-κB p65, and p-IκBα of tumor sections of different treated groups. Significant differences are indicated as follows: ***P < 0.001.