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Fibronectin type III domain-containing 5 (Fndc5) expression and irisin levels in control (Ctrl; n = 8) and Fndc5 overexpression (OE; n = 8) animals. A: Fndc5 mRNA in tibialis cranialis (TC) muscle (P = 0.014). B: Fndc5 protein expression in TC (P = 0.036). C: plasma irisin concentration (P = 0.049). Values are means ± SD. Statistical analysis was via unpaired t test. *P < 0.05 vs. Ctrl. Molecular mass is in kilodaltons.
