Fig. 1.
Chronic inhibition of IL-17 in reduced uterine perfusion pressure (RUPP) rats normalizes circulating and placental IL-17 and T-helper (TH)17 cell populations. A subset of pregnant rats undergoing the RUPP procedure also received a miniosmotic pump infusing 100 pg/day of a soluble IL-17 receptor (IL-17RC) into the intraperitoneal cavity from gestational days (GD) 14–19. On GD19, blood and placentas were collected, processed, and analyzed via ELISA and flow cytometry to obtain levels of circulating (A) and placental (B) IL-17 and percentages of circulating (C) and placental (D) TH17 cells. Normal pregnant (NP): n = 9 rats; RUPP: n = 9 rats RUPP + IL-17RC: n = 9 rats. All data are expressed as means ± SD. Statistical analysis was performed using one-way ANOVA with multiple comparisons followed by Tukey’s post hoc correction. *P < 0.05 vs. NP; #P < 0.05 vs. RUPP.