Fig. 3.
Effects of BMAL1 deletion and/or chronic SR-9009 administration on cardiac gene expression. Cardiomyocyte-specific BMAL1-knockout (CBK) and littermate control (CON) mice were challenged with either SR-9009 (SR; 100 mg·kg−1·day−1 ip) or vehicle (Veh) at zeitgeber time (ZT)9 for 8 consecutive days, followed by heart isolation 6 h after the last administration (i.e., ZT15). A: pdk4 expression was assessed by RT-PCR. B: bmal1 (i), clock (ii), per1 (iii), cry2 (iv), rev-erbα (v), rev-erbβ (vi), dbp (vii), and e4bp4 (viii) were assessed by RT-PCR. Data are reported as means ± SE for 13–15 mice/experimental group. Main effects for either SR-9009, genotype, and/or interaction are reported at the top of each graph. *P < 0.05 for CON (●) vs. CBK (red squares) within a treatment group. $P < 0.05 for vehicle vs. SR-9009 administration within a genotype.