Fig. 5.

Effects of BMAL1 deletion and/or SR-9009 administration on mitochondrial parameters. Cardiomyocyte-specific BMAL1-knockout (CBK) and littermate control (CON) mice were challenged with either SR-9009 (SR; 100 mg·kg⁻¹·day⁻¹ ip) or vehicle (Veh) at zeitgeber time (ZT)9 for 8 consecutive days, followed by heart isolation 6 h after the last administration (i.e., ZT15). A: mitochondrial complex protein levels were assessed by Western blotting (and normalized to amido black). B: mitochondrial complex activities were assessed by following oxygen consumption rates in the presence of specific substrates. C: citrate synthase activity was assessed spectrophotometrically. Data are reported as means ± SE for 6–8 mice/experimental group. Main effects for either genotype or SR are reported at the top of each graph. ●, CON; red squares, CBK.