Fig. 6.

Dynamic changes in mitochondrial signal in large vessels. Time-lapse images of a large brain vessel show changes in mitochondrial signal. Red shows Rhodamine dextran-labeled plasma, whereas green shows endothelium-specific mito-Dendra2 signal. Zero or first time-point images are labeled as “1st #1–10.” Second set of images are noted as “2nd #1–10.” The numbers after the # sign are indicative of image frames. These images were split in half, resulting in the following subgroups: “1st #1–5” and “1st #6–10” and “2nd #1–5” and “2nd #6–10.” Gray boxes indicate the area with apparent changes in mitochondrial signal. Boxed area was enlarged and binarized images show changes in signal between the 2-imaging time points.