Table 1.
Primers used for real-time qPCR and antibodies used for Western blot analysis
| No. | Gene | Primer |
|---|---|---|
| 1 | Srebp2 | F-tggatgacgcaaaggtcaa R-caggaaggtgaggacacataag |
| 2 | Hmgcs | F- gcatatatagcaatgtctcctgcaa R- gccgtgaactgggtcgaa |
| 3 | Hmgcr | F- cttgtggaatgccttgtgattg R-agcccgaagcagcacatgat |
| 4 | Bsep | F- aaattcaagcaggctcaagacaa R-taccaactggggtgctctttt |
| 5 | Cyp8b1 | F-tgaacgacttcttggcttcc R-cctgtttctgggtcctcttattc |
| 6 | Cyp7a1 | F-tcacaaactccctgtcatacc R-catcacttgggtctatgcttct |
| 7 | Cyp27a1 | F-gcctcacctatgggatcttca R-tcaaagcctgacgcagatg |
| 8 | Abcg8 | F-ctgtacactgctggtccttatt R-caggtttgtcagccagtagat |
| 9 | Abcg5 | F-cttacccacggttcctttca R-actgcctgcttattcctcatta |
| 10 | Abca1 | F-gggtggtgttcttcctcattac R-cacatcctcatcctcgtcattc |
| 11 | Asbt | F-attggatagatggcgacatgg R-gtgtagacgaagaggcaaagag |
| 12 | Shp | F-ctgaagggcacgatcctct R-gcctcctgttgcaggtgt |
| 13 | Npc1l1 | F-tggactggaaggaccatttcc R-gtgccccgtagtcagctat |
| 14 | Ostα | F-gctcacctccctactcttcta R-tacccaaccttgtcgtctttc |
| 15 | Ostβ | F-caggaactgctggaagaaatg R-ccaggaccaggatggaataa |
| 16 | Fxr | F-gggcagaatctggatttgga R-gtagaagcccaggttggaatag |
| 17 | Fgf15 | F-agatggtgcttcatggatctg R-catataccgggctgattcgctac |
| 18 | Ibabp | F-tgagagtgagaagaattacgatgag R-ctgacctctgtgatgatcttg |
| 19 | Gapdh | F-cctcgtcccgtagacaaaatg R- tgaaggggtcgttgatggc |
Abca1, Abcg5, and Abcg8, ATP-binding cassette transporters; Asbt, apical sodium bile acid transporter; Bsep, bile salt export pump; Cyp7a1, Cyp8b1, and Cyp27a1, bile acid modifying cytochrome-P450 family members; Fgf15, fibroblast growth factor 15; Fxr, farnesoid X receptor; Hmgcr, HMG-CoA reductase; Hmgcs, HMG-CoA synthase; Ibabp, ileal bile acid-binding protein; Npc1l1, Niemann-pick C1-like 1; Ostα and Ostβ; organic solute transporter α and β; Shp, small heterodimeric partner; Srebp2, sterol regulatory-binding protein 2. F, forward; R, reverse.