Table 2.
Primary and secondary antibodies
| No. | Antibody | Vendor | Catalog No. |
|---|---|---|---|
| Primary antibody information | |||
| 1 | ABCA1 | Novus-Biological | NB400-105 |
| 2 | NPC1L1 | Gift from Dr. Joyce Repa (Univ. of Texas–Southwestern Medical Center | Not applicable |
| 3 | HMGCOA-reductase | Gift from Dr. Russell DeBose-Boyd (Univ. of Texas–Southwestern Medical Center) | Not applicable |
| 4 | CYP7A1 | Abcam | Ab65596 |
| 5 | Calnexin | Cell Signaling Technologies | (C5C9)#2679 |
| 6 | GAPDH | Cell Signaling Technologies | (D16H11)#8884 |
| Secondary antibody information | |||
| 1 | Anti-rabbit HRP-conjugated | Cell Signaling Technologies | 7074 |
| 2 | Anti-mouse HRP-conjugated | Cell Signaling Technologies | 7076 |
ABCA1, ATP-binding cassette transporter; CYP7A1, bile acid modifying cytochrome-P450 family member; NPC1L1, Niemann-pick C1-like 1.