Fig. 4.

Cisplatin treatment alters inflammatory cytokine production by kidney double negative (DN) T cells. A: we measured proinflammatory cytokines interferon (IFN)-γ, IL-17A, and TNF-α and anti-inflammatory cytokine IL-10 to explore cisplatin effects on kidney DN T cell effector function. Cisplatin treatment resulted in a significant increase in IL-10 and decrease in IFN-γ, TNF-α, and IL-17A at 24 h. B: there was no difference in the percentages of CD4⁺ and CD8⁺ T cell cytokine profiles between cisplatin-treated and control groups at any time point studied. C: the absolute numbers of CD4⁺ and CD8⁺ T cells producing proinflammatory cytokines (IFN-γ, TNF-α, and IL-17A) were, however, significantly reduced after cisplatin treatment. Data are presented as means ± SE from at least 5 independent experiments (n = 5 mice). *P < 0.05; **P < 0.01. SSC, side scatter; ns, not significant.