Fig. 5.

Cisplatin affects both programmed cell death (PD)-1⁺ and natural killer (NK)1.1⁺ subsets of kidney double negative (DN) T cells. A: to understand the effect of cisplatin treatment on PD-1⁺ and NK1.1⁺ subsets of kidney DN T cells, we isolated kidney mononuclear cells from cisplatin-treated and vehicle-treated mice and assessed the frequency of different subsets with flow cytometry. The frequency and absolute numbers of the PD-1⁺ DN T cell subset significantly declined after cisplatin treatment. There was no change in the frequency of PD-1⁺CD4⁺ and PD-1⁺CD8⁺ T cells; however, the absolute numbers were significantly decreased. Cisplatin treatment did not affect the frequency of the NK1.1⁺ subset in DN, CD4⁺, or CD8⁺ T cells; however, the absolute number of NK1.1⁺ DN T cells decreased significantly at 24 and 72 h after cisplatin. The frequency of dual PD-1⁺NK1.1⁺ DN T cells increased at 72 h after cisplatin treatment. Data are presented as means ± SE from at least 5 independent experiments (n = 5 mice). *P < 0.05; **P < 0.01.