Fig. 7.

Double negative (DN) T cells reduce cisplatin (Cis)-induced programmed cell death ligand-1 (PD-L1) expression in proximal tubular epithelial cells (PTECs). A: we assessed the effect of DN T cell coculture on PTECs and quantified the expression of PD-L1. There was an increase in PD-L1 expression in PTECs after cisplatin treatment at 72 h. PTECs cocultured with DN T cells showed a trend toward reducing PD-L1 levels compared with PTECs cultured alone, but it was not statistically significant. B: to further confirm whether there was a reduction in PD-L1 levels after coculture, we directly quantified PD-L1 in PTECs using Western blot analysis. We observed a significant reduction in PD-L1 protein in PTECs in response to cisplatin treatment when PTECs were cocultured with DN T cells. Data are presented as means ± SE. *P < 0.05; **P < 0.01.