Table 3.
Raw values for changes in urine Na+ and K+ after 6-h diuretic treatment
| Vehicle | Furosemide | Vehicle | Hydrochlorothiazide | Vehicle | Amiloride | |
|---|---|---|---|---|---|---|
| Low K+ | ||||||
| UNa, mmol/L | 106 (5) n = 11 |
82 (6) n = 12 |
101 (8) n = 6 |
189 (10)† n = 6 |
106 (5) n = 11 |
142 (13) n = 12 |
| UNa × V, mmol/6 h | 0.113 (0.015) n = 11 |
0.136 (0.013) n = 12 |
0.043 (0.007) n = 6 |
0.177 (0.028)† n = 6 |
0.116 (0.013) n = 11 |
0.142 (0.023) n = 12 |
| UK, mmol/L | 1.636 (0.157) n = 11 |
2.167 (0.428) n = 12 |
1.333 (0.198) n = 6 |
1.067 (0.169) n = 6 |
1.38 (0.190) n = 11 |
1.23 (0.187) n = 12 |
| UK × V, mmol/6 h | 0.002 (0.000) n = 11 |
0.004 (0.000) n = 12 |
0.0005 (0.0001) n = 6 |
0.0011 (0.0003) n = 6 |
0.002 (0.000) n = 11 |
0.002 (0.001) n = 12 |
| K+ basic | ||||||
| UNa, mmol/L | 162 (12) n = 9 |
131 (8) n = 11 |
158 (14) n = 5 |
172 (9) n = 5 |
127 (6) n = 10 |
308 (39)† n = 10 |
| UNa × V mmol/6 h | 0.081 (0.014) n = 9 |
0.162 (0.020)† n = 11 |
0.063 (0.012) n = 5 |
0.071 (0.017) n = 5 |
0.072 (0.013) n = 10 |
0.108 (0.022) n = 10 |
| UK, mmol/L | 447 (42) n = 9 |
198 (28)† n = 11 |
373 (41) n = 5 |
387 (68) n = 5 |
418 (42) n = 10 |
277 (41)† n = 10 |
| UK × V, mmol/6 h | 0.229 (0.042) n = 9 |
0.274 (0.055) n = 11 |
0.146 (0.028) n = 5 |
0.153 (0.033) n = 5 |
0.246 (0.053) n = 10 |
0.095 (0.021)† n = 10 |
| KCl | ||||||
| UNa, mmol/L | 90 (4) n = 12 |
84 (6) n = 12 |
106 (10) n = 4 |
124 (28) n = 6 |
87 (4) n = 12 |
258 (15)† n = 12 |
| UNa × V, mmol/6 h | 0.058 (0.008) n = 12 |
0.140 (0.014)† n = 12 |
0.069 (0.014) n = 4 |
0.098 (0.025) n = 6 |
0.073 (0.009) n = 12 |
0.126 (0.018) n = 12 |
| UK, mmol/L | 482 (13) n = 12 |
153 (14)† n = 12 |
560 (73) n = 4 |
479 (96) n = 6 |
493 (16) n = 12 |
268 (47)† n = 12 |
| UK × V, mmol/6 h | 0.308 (0.040) n = 12 |
0.278 (0.041) n = 12 |
0.364 (0.080) n = 4 |
0.371 (0.089) n = 6 |
0.408 (0.048) n = 12 |
0.135 (0.025)† n = 12 |
Values are means (SE); n, number of mice. Mice were treated with varying K+ diets for 10 days and then injected with a diuretic [either furosemide (30 mg/kg), hydrochlorothiazide (25 mg/kg), or amiloride (5 mg/kg)], and urine was collected after 6 h. UNa × V and UK × V, urinary Na+ and K+ excretion. Results were compared with 6-h urine collected from a sham vehicle-injected mouse on an alternate day. Two-way ANOVA with Sidak’s post hoc analysis was used.
†
P ≤ 0.01, significant difference between vehicle and diuretic, respectively.