Table 3.
Basal physiological parameters in young adult control and KS-BMAL1 KO mice measured from samples collected at noon (Zeitgeber 6)
| Parameter (Noon) | Control Mice | KS-BMAL1 KO Mice |
|---|---|---|
| Body weight, g | ||
| Male | 31.7 ± 0.8 (12) | 32.2 ± 0.7 (12) |
| Female | 21.7 ± 0.6 (12)‡ | 22.2 ± 0.7 (12)‡ |
| Blood Na+, mmol/L | ||
| Male | 145.5 ± 0.4 (8) | 145.9 ± 0.6 (4) |
| Female | 146.5 ± 0.7 (4) | 145.6 ± 0.6 (7) |
| Blood K+, mmol/L | ||
| Male | 4.9 ± 0.09 (6) | 4.5 ± 0.11 (5) |
| Female | 5.8 ± 0.2 (4)‡ | 5.8 ± 0.3 (6)‡ |
| Blood bicarbonate, mmol/L | ||
| Male | 15.5 ± 0.4 (8) | 17.2 ± 1.2 (4)† |
| Female | 16.1 ± 0.7 (4) | 19.3 ± 0.6 (7)*† |
| Blood pH | ||
| Male | 7.4 ± 0.02 (8) | 7.4 ± 0.02 (4) |
| Female | 7.4 ± 0.03 (4) | 7.4 ± 0.02 (7) |
| Hematocrit | ||
| Male | 38.6 ± 0.3 (8) | 42.0 ± 4.4 (4) |
| Female | 53.75 ± 1.7 (4)‡ | 54.4 ± 0.9 (7)‡ |
| Urine volume, g/24 h | ||
| Male | 4.2 ± 0.5 (6) | 4.9 ± 0.3 (5) |
| Female | 1.8 ± 0.2 (6)‡ | 2.5 ± 0.4 (6)‡ |
Values are means ± SE with numbers of animals per group shown in parentheses. Arterial blood samples were run through a blood gas analyzer to determine Na+, K+, bicarbonate, pH, and hematocrit. Serum K+ was measured via flame photometry. KS-BMAL1 KO mice, kidney-specific brain and muscle ARNT-like 1 knockout mice. Two-way ANOVA with Tukey multiple-comparisons test was used for statistical analysis.
*
Significantly differenct vs. control mice of the same sex (Tukey);
†
significantly different vs. control mice;
‡
significantly different vs. male mice.