Fig. 2.

Store-operated Ca²⁺ entry increased secretion and production of interleukin-6 (IL-6) protein by human mesangial cells (HMCs). A: ELISA showing IL-6 concentrations in HMC culture media. HMCs were growth arrested with serum-free DMEM overnight. One group had no treatment (NT), and the other two groups were treated with thapsigargin (TG; 1 μM) with or without GSK-7975A (GSK; 10 μM) for 15 h. Supernatants of the culture media were collected at the end of treatment. *P < 0.05. B: representative immunoblots showing the effect of Store-operated Ca²⁺ entry on IL-6 protein content in cultured HMCs. Serum-deprived HMCs had no treatment (NT) or were treated with TG (1 μM) with or without GSK (10 μM) for 15 h. α-Tubulin was used as the loading control. C: summary data from the experiment shown in B. *P < 0.05 vs. NT and TG + GSK. n = 5, where n indicates the number of independent experiments. D: representative immunoblots showing the effect of tunicamycin on IL-6 protein content in cultured HMCs. Serum-deprived HMCs had no treatment (NT) or were treated with DMSO (1:1,000) or tunicamycin (10 μM) for 15 h. α-Tubulin was used as the loading control. E: summary data from the experiment shown in B. n = 5, where n indicates the number of independent experiments.