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. 2020 May 11;318(6):F1478–F1488. doi: 10.1152/ajprenal.00043.2020

Fig. 7.

Fig. 7.

In vivo knockdown of Orai1 in mesangial cells decreased glucagon-like peptide-1 receptor (GLP-1R) content in glomeruli in mice. A: representative images of immunohistochemical staining of GLP-1R in kidneys of mice treated with nanoparticle (NP)/scrambled siRNA or NP/siRNA for Orai1 (siOrai1). GLP-1R staining is indicated by the brown color, whereas nuclei are blue. Arrows indicate glomeruli. Original magnification: ×200. B: enlarged images of the region indicated by the red dashed boxes in A. C: integrated density of GLP-1R staining averaged from 3 NP/scrambled siRNA- and 3 NP/siOrai1-treated mice. Numbers in parentheses under each bar represent the number of glomeruli analyzed from 5 sections/kidney. *P < 0.05 compared with NP/scrambled siRNA. Con, control.